Biophotonics across Energy, Space and Time
Mesoscale Biophotonics: Wide-Field Functional Imaging
Wide-field functional imaging

Mesoscale Biophotonics refers to a broad class of optical methods that utilize the interaction of photons with cells and tissues on the sub-millimeter to millimeter spatial scales to provide wide-field functional images of cell and tissue structure, composition, and flow. Wide-field functional imaging methods including Optical Coherence and Doppler tomography (Chen), Laser Speckle contrast Imaging (Choi), Spatial Frequency-Domain Imaging (Durkin, Frostig, Tromberg), and Bioluminescent Imaging (Prescher) have emerged within the last decade. Participating BEST faculty have been at the forefront in establishing state-of-the-art capailities of these methods. Their application in various biological systems has led to a deeper understanding of cellular and tissue processes and addressed critical biomedical needs.

The technology development has enabled high spatial and temporal resolutions to examine both fast (i.e., ms timescale) and localized (i.e., tens of micrometers to millimeters) events at depths of several millimeters in thick tissues. A major objective is to develop technologies for the quantitative imaging of tissue structure, composition, metabolism, and flow with scalable resolution and depth sectioning. Such methods encompass the imaging of native and exogenous contrast agents through the measurement of reflectance, fluorescence, and/or bioluminescent signals. These capabilities will provide exciting opportunities to BEST trainees to examine biological processes on the tissue level to understand complex cellular systems and disease etiology as well as to develop novel and improved imaging and diagnostic tools to advance biomedical practice.

BEST Faculty for Wide-Field Functional Imaging

Chen (Biomedical Engineering & Surgery), Choi (Biomedical Engineering & Surgery), Durkin (Surgery), Frostig (Neurobiology & Behavior, Biomedical Engineering), Gratton (Biomedical Engineering, Physics & Astronomy), Prescher (Chemistry, Molecular Biology & Biochemistry, Pharmaceutical Sciences), Tromberg (Biomedical Engineering, Surgery)